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ebf3 igg  (Novus Biologicals)


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    Structured Review

    Novus Biologicals ebf3 igg
    Validation of VMP- and SU-specific transcript expression in female and male P0 rat tissues. Quantitative real-time PCR (qPCR) showed significantly elevated levels of both control (Fgf10, Ptn and Scube1) and candidate <t>(Ebf3,</t> Gfra3, Nmur2, Rspo2, Scara5, Slc26a7, Robo1 and Meis2) VMP-specific transcripts versus SU. Fgf10, Ptn, Scube1, Ebf3, Gfra3, Scara5 and Meis2 were expressed in VP and DP, while Rspo2, Nmur2 and Slc26a7 showed low expression in VP and DP. SU candidate transcripts Anxa1, Enpp2 and Unc5b were enriched versus VMP. Data is represented as mean fold difference to VMP ± SD of duplicate biological replicates and duplicate technical replicates (n = 4). Significance was detected using One-way ANOVA with TUKEY multiple comparison *p < 0.05. Figures in red indicate fold difference compared to VMP.
    Ebf3 Igg, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ebf3 igg/product/Novus Biologicals
    Average 90 stars, based on 5 article reviews
    ebf3 igg - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Identification of genes expressed in a mesenchymal subset regulating prostate organogenesis using tissue and single cell transcriptomics"

    Article Title: Identification of genes expressed in a mesenchymal subset regulating prostate organogenesis using tissue and single cell transcriptomics

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-16685-8

    Validation of VMP- and SU-specific transcript expression in female and male P0 rat tissues. Quantitative real-time PCR (qPCR) showed significantly elevated levels of both control (Fgf10, Ptn and Scube1) and candidate (Ebf3, Gfra3, Nmur2, Rspo2, Scara5, Slc26a7, Robo1 and Meis2) VMP-specific transcripts versus SU. Fgf10, Ptn, Scube1, Ebf3, Gfra3, Scara5 and Meis2 were expressed in VP and DP, while Rspo2, Nmur2 and Slc26a7 showed low expression in VP and DP. SU candidate transcripts Anxa1, Enpp2 and Unc5b were enriched versus VMP. Data is represented as mean fold difference to VMP ± SD of duplicate biological replicates and duplicate technical replicates (n = 4). Significance was detected using One-way ANOVA with TUKEY multiple comparison *p < 0.05. Figures in red indicate fold difference compared to VMP.
    Figure Legend Snippet: Validation of VMP- and SU-specific transcript expression in female and male P0 rat tissues. Quantitative real-time PCR (qPCR) showed significantly elevated levels of both control (Fgf10, Ptn and Scube1) and candidate (Ebf3, Gfra3, Nmur2, Rspo2, Scara5, Slc26a7, Robo1 and Meis2) VMP-specific transcripts versus SU. Fgf10, Ptn, Scube1, Ebf3, Gfra3, Scara5 and Meis2 were expressed in VP and DP, while Rspo2, Nmur2 and Slc26a7 showed low expression in VP and DP. SU candidate transcripts Anxa1, Enpp2 and Unc5b were enriched versus VMP. Data is represented as mean fold difference to VMP ± SD of duplicate biological replicates and duplicate technical replicates (n = 4). Significance was detected using One-way ANOVA with TUKEY multiple comparison *p < 0.05. Figures in red indicate fold difference compared to VMP.

    Techniques Used: Biomarker Discovery, Expressing, Real-time Polymerase Chain Reaction, Control, Comparison

    Immunohistochemistry of Ebf3 and Meis2 in female VMP and male prostate. To examine the distribution of Ebf3 and Meis2 in mesenchymal subsets in VMP and ventral prostate (VP) we performed immunohistochemistry using P0 rat female and male reproductive tracts. Panels ( a–d ) show Ebf3 expression; in female ( a and c ) and male ( b and d ). Ebf3 showed a highly selective distribution within VMP and VP mesenchyme, and was absent from smooth muscle (SM) and the urethra ( c , a and b ). Panels ( e–h ) show Meis2 distribution; in female ( e and g ) and male ( f and h ) mesenchyme. Urethral epithelia (URE) and prostatic epithelia (E) were negative for both Ebf3 and Meis2. Scale bars ( a , b , e and f ) = 600 µm. Scale bars ( c , d , g and h ) = 300 µm.
    Figure Legend Snippet: Immunohistochemistry of Ebf3 and Meis2 in female VMP and male prostate. To examine the distribution of Ebf3 and Meis2 in mesenchymal subsets in VMP and ventral prostate (VP) we performed immunohistochemistry using P0 rat female and male reproductive tracts. Panels ( a–d ) show Ebf3 expression; in female ( a and c ) and male ( b and d ). Ebf3 showed a highly selective distribution within VMP and VP mesenchyme, and was absent from smooth muscle (SM) and the urethra ( c , a and b ). Panels ( e–h ) show Meis2 distribution; in female ( e and g ) and male ( f and h ) mesenchyme. Urethral epithelia (URE) and prostatic epithelia (E) were negative for both Ebf3 and Meis2. Scale bars ( a , b , e and f ) = 600 µm. Scale bars ( c , d , g and h ) = 300 µm.

    Techniques Used: Immunohistochemistry, Expressing

    Summary of primers used for qPCR.
    Figure Legend Snippet: Summary of primers used for qPCR.

    Techniques Used:



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    Validation of VMP- and SU-specific transcript expression in female and male P0 rat tissues. Quantitative real-time PCR (qPCR) showed significantly elevated levels of both control (Fgf10, Ptn and Scube1) and candidate <t>(Ebf3,</t> Gfra3, Nmur2, Rspo2, Scara5, Slc26a7, Robo1 and Meis2) VMP-specific transcripts versus SU. Fgf10, Ptn, Scube1, Ebf3, Gfra3, Scara5 and Meis2 were expressed in VP and DP, while Rspo2, Nmur2 and Slc26a7 showed low expression in VP and DP. SU candidate transcripts Anxa1, Enpp2 and Unc5b were enriched versus VMP. Data is represented as mean fold difference to VMP ± SD of duplicate biological replicates and duplicate technical replicates (n = 4). Significance was detected using One-way ANOVA with TUKEY multiple comparison *p < 0.05. Figures in red indicate fold difference compared to VMP.
    Ebf3 Igg, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ebf3 igg/product/Novus Biologicals
    Average 90 stars, based on 1 article reviews
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    Image Search Results


    Validation of VMP- and SU-specific transcript expression in female and male P0 rat tissues. Quantitative real-time PCR (qPCR) showed significantly elevated levels of both control (Fgf10, Ptn and Scube1) and candidate (Ebf3, Gfra3, Nmur2, Rspo2, Scara5, Slc26a7, Robo1 and Meis2) VMP-specific transcripts versus SU. Fgf10, Ptn, Scube1, Ebf3, Gfra3, Scara5 and Meis2 were expressed in VP and DP, while Rspo2, Nmur2 and Slc26a7 showed low expression in VP and DP. SU candidate transcripts Anxa1, Enpp2 and Unc5b were enriched versus VMP. Data is represented as mean fold difference to VMP ± SD of duplicate biological replicates and duplicate technical replicates (n = 4). Significance was detected using One-way ANOVA with TUKEY multiple comparison *p < 0.05. Figures in red indicate fold difference compared to VMP.

    Journal: Scientific Reports

    Article Title: Identification of genes expressed in a mesenchymal subset regulating prostate organogenesis using tissue and single cell transcriptomics

    doi: 10.1038/s41598-017-16685-8

    Figure Lengend Snippet: Validation of VMP- and SU-specific transcript expression in female and male P0 rat tissues. Quantitative real-time PCR (qPCR) showed significantly elevated levels of both control (Fgf10, Ptn and Scube1) and candidate (Ebf3, Gfra3, Nmur2, Rspo2, Scara5, Slc26a7, Robo1 and Meis2) VMP-specific transcripts versus SU. Fgf10, Ptn, Scube1, Ebf3, Gfra3, Scara5 and Meis2 were expressed in VP and DP, while Rspo2, Nmur2 and Slc26a7 showed low expression in VP and DP. SU candidate transcripts Anxa1, Enpp2 and Unc5b were enriched versus VMP. Data is represented as mean fold difference to VMP ± SD of duplicate biological replicates and duplicate technical replicates (n = 4). Significance was detected using One-way ANOVA with TUKEY multiple comparison *p < 0.05. Figures in red indicate fold difference compared to VMP.

    Article Snippet: Immunostaining of Ebf3 and Meis2 on serial sections of female and male rat P0 urogenital sinus tissue (isolated as per ) was performed as per using Ebf3 IgG (Clone 8D6, mouse monoclonal, Novus Biologicals, Littleton, Colorado, USA; dilution 1:1000) and Meis2 IgG (Clone 63-T, mouse monoclonal, Santa Cruz Biotechnology, Santa Cruz, USA; dilution 1:750).

    Techniques: Biomarker Discovery, Expressing, Real-time Polymerase Chain Reaction, Control, Comparison

    Immunohistochemistry of Ebf3 and Meis2 in female VMP and male prostate. To examine the distribution of Ebf3 and Meis2 in mesenchymal subsets in VMP and ventral prostate (VP) we performed immunohistochemistry using P0 rat female and male reproductive tracts. Panels ( a–d ) show Ebf3 expression; in female ( a and c ) and male ( b and d ). Ebf3 showed a highly selective distribution within VMP and VP mesenchyme, and was absent from smooth muscle (SM) and the urethra ( c , a and b ). Panels ( e–h ) show Meis2 distribution; in female ( e and g ) and male ( f and h ) mesenchyme. Urethral epithelia (URE) and prostatic epithelia (E) were negative for both Ebf3 and Meis2. Scale bars ( a , b , e and f ) = 600 µm. Scale bars ( c , d , g and h ) = 300 µm.

    Journal: Scientific Reports

    Article Title: Identification of genes expressed in a mesenchymal subset regulating prostate organogenesis using tissue and single cell transcriptomics

    doi: 10.1038/s41598-017-16685-8

    Figure Lengend Snippet: Immunohistochemistry of Ebf3 and Meis2 in female VMP and male prostate. To examine the distribution of Ebf3 and Meis2 in mesenchymal subsets in VMP and ventral prostate (VP) we performed immunohistochemistry using P0 rat female and male reproductive tracts. Panels ( a–d ) show Ebf3 expression; in female ( a and c ) and male ( b and d ). Ebf3 showed a highly selective distribution within VMP and VP mesenchyme, and was absent from smooth muscle (SM) and the urethra ( c , a and b ). Panels ( e–h ) show Meis2 distribution; in female ( e and g ) and male ( f and h ) mesenchyme. Urethral epithelia (URE) and prostatic epithelia (E) were negative for both Ebf3 and Meis2. Scale bars ( a , b , e and f ) = 600 µm. Scale bars ( c , d , g and h ) = 300 µm.

    Article Snippet: Immunostaining of Ebf3 and Meis2 on serial sections of female and male rat P0 urogenital sinus tissue (isolated as per ) was performed as per using Ebf3 IgG (Clone 8D6, mouse monoclonal, Novus Biologicals, Littleton, Colorado, USA; dilution 1:1000) and Meis2 IgG (Clone 63-T, mouse monoclonal, Santa Cruz Biotechnology, Santa Cruz, USA; dilution 1:750).

    Techniques: Immunohistochemistry, Expressing

    Summary of primers used for qPCR.

    Journal: Scientific Reports

    Article Title: Identification of genes expressed in a mesenchymal subset regulating prostate organogenesis using tissue and single cell transcriptomics

    doi: 10.1038/s41598-017-16685-8

    Figure Lengend Snippet: Summary of primers used for qPCR.

    Article Snippet: Immunostaining of Ebf3 and Meis2 on serial sections of female and male rat P0 urogenital sinus tissue (isolated as per ) was performed as per using Ebf3 IgG (Clone 8D6, mouse monoclonal, Novus Biologicals, Littleton, Colorado, USA; dilution 1:1000) and Meis2 IgG (Clone 63-T, mouse monoclonal, Santa Cruz Biotechnology, Santa Cruz, USA; dilution 1:750).

    Techniques: